91��������

Thurs 24 Jul

2:00 91�������ד 5:00       Student check-in (Swope)

6:00 91�������ד 7:00       Dinner (Swope)

7:00 91�������ד 7:15       Welcome to the Zebrafish Course: Tom Schilling & Andi Pauli (Speck Auditorium)

7:15 91�������ד 8:15       Overview: Zebrafish Development and Genetics: Lila Solnica-Krezel (Speck Auditorium)

8:15 91�������ד 9:15       Student 3-min oral presentations (Speck Auditorium)

9:30 91�������ד 10:30     Set up posters and locate individual student stations in lab (Loeb 260)

Fri 25 Jul

8:30 91�������ד 8:45       Welcome to the MBL: Linda Hyman, Director of Education (Loeb 260)

8:45 91�������ד 9:15       Introduction to stereoscopes, iPad/camera & server: Dave Raible & Faculty (Loeb 260)

9:15 91�������ד 12:00     Pit Talk & Lab: Zebrafish Development: Lila Solnica-Krezel & Dave Raible (Loeb 260)

12:00 91�������ד 1:30     Faculty (Tom Schilling & Andi Pauli) lunch with students (Swope)

1:30 91�������ד 2:30       Lab: Zebrafish Development, continued: Lila Solnica-Krezel & Dave Raible (Loeb 260)

2:30 91�������ד 3:30       Lecture: Introduction to Microscopy: Andres Collazo (Loeb 260)

3:30 91�������ד 6:00       Hands-on microscopy training: Andres Collazo, Jim Fadool, Dave Raible, & Tom Schilling (Loeb 260)

7:30 91�������ד 9:00       Research Lectures & Discussion: Dave Raible & Lila Solnica-Krezel (Speck Auditorium)

9:00 91�������ד 9:30       Follow up on embryos and wrap-up from morning session: Lila Solnica-Krezel

9:30 91�������ד 11:00     91�������טDiSci91�������י (science discussions with speakers, faculty, and at posters)

Sat 26 Jul

8:30 91�������ד 9:30       Pit Talk: Larval anatomy: Tom Schilling (Loeb 260)

9:30 91�������ד 12:00     Lab: Larval anatomy tour with fluorescent transgenics: Andres Collazo, Jim Fadool, Dave Raible, & Tom Schilling (Loeb 260)

12:00 91�������ד 1:30     Faculty (Dave Raible & Lila Solnica-Krezel) lunch with students (Swope)

1:30 91�������ד 2:30       Workshop: Developmental Genetics: Mary Mullins (Candle House 104)

2:30 91�������ד 3:30       Lecture: Light Sheet Microscopy: Jan Huisken (Speck Auditorium)

3:30 91�������ד 4:30       Lab: imaging and mounting transgenics: Dave Raible, & Tom Schilling (Loeb 260)

4:30 91�������ד 6:00       Time-lapse microscopy: Andres Collazo & Jim Fadool (Group 1)

4:30 91�������ד 6:00       Time-lapse microscopy: Jan Huisken and Dane Maxfield (Group 4)

4:30 91�������ד 6:00       Groups 2 and 3 work on T-shirt design

7:30 91�������ד 9:00       Research Lectures & Discussion: Mary Mullins & Alex Schier (Speck Auditorium)

9:00 91�������ד 11:00     91�������טDiSci91�������י (continued science discussions with speakers, faculty, and at posters)

Sun 27 Jul

8:30 91�������ד 9:30       Wrap-up: Larval Anatomy: Tom Schilling & Dave Raible (Loeb 263)

9:30 91�������ד 12:00     Pit Talk & Lab: Mounting, imaging, & analysis of mRNA and protein in embryos and larvae: Kara Cerveny & Fabienne Poulain (Loeb 260)

12:00 91�������ד 1:30     Faculty (Jan Huisken & Alex Schier) lunch with students (Swope)

1:30 91�������ד 4:00       Lab: Mounting, imaging, & analysis of mRNA and protein, including wrap up: Kara Cerveny & Fabienne Poulain (Loeb 260)

4:00 91�������ד 5:00       Workshop: Testing Gene Function & Designer Genes: Mary Mullins (Candle House 104)

5:00 91�������ד 6:00       Time-lapse microscopy: Andres Collazo & Jim Fadool (Group 2)

5:00 91�������ד 6:00       Time-lapse microscopy: Jan Huisken and Dane Maxfield (Group 3)

5:00 91�������ד 6:00       Groups 1 and 4 work on T-shirt design

7:30 91�������ד 9:00       Research Lectures & Discussion: Maura McGrail & Becky Burdine (Speck Auditorium)

9:00 91�������ד 11:00     91�������טDiSci91�������י (continued w speakers and all faculty present) + T shirt design

Mon 28 Jul

8:30 91�������ד 12:00     Pit Talk & Lab: mRNA and CRISPR injections: Andi Pauli & Becky Burdine (Loeb 260)

12:00 91�������ד 1:30     Faculty (Mary Mullins & Fabienne Poulain) lunch with students (Swope)

1:30 91�������ד 3:30       Workshop: Zebrafish Genome Literacy: Maura McGrail (Bay Reading Room in Lillie)

3:30 91�������ד 4:30       Workshop: When should I (not) use zebrafish as a model?: Alex Schier (Loeb 263)

4:30 91�������ד 6:00       Time-lapse microscopy: Andres Collazo & Jim Fadool (Group 3)

4:30 91�������ד 6:00       Time-lapse microscopy: Jan Huisken and Dane Maxfield (Group 1)

4:30 91�������ד 6:00       Groups 2 and 4 work on T-shirt design

7:30 91�������ד 9:00       Research Lectures & Discussion: Holger Knaut & Fabienne Poulain (Speck Auditorium)

9:00 91�������ד 9:45       Wrap-up mRNA injected embryos: Andi Pauli & Becky Burdine (Loeb 260); start 2 time-lapses with germline-labelled or fluorescently injected embryos

9:45 91�������ד 11:00     91�������טDiSci91�������י (continued w speakers and all faculty present)

11:00                T-shirt design is due by 11:00 pm on July 28

Tues 29 Jul

8:30 91�������ד 12:00     Pit Talk & Lab: Morpholino & CRISPR injections: Andi Pauli, Becky Burdine, & Holger Knaut (Loeb 260)

11:00 91�������ד 12:00   Needle pulling demo: Adair Oesterle (Sutter Instruments) (Loeb 260)

12:00 91�������ד 1:30     Faculty (Maura McGrail & Becky Burdine) lunch with students (Swope)

1:30 91�������ד 4:00       Workshop: CRISPR Design: Maura McGrail (Bay Reading Room in Lillie)

4:00 91�������ד 5:30       Workshop: Single-cell and Spatial Transcriptomics: Alex Schier (Candle House 104)

5:30 91�������ד 6:00       Check on mRNA injected embryos: Andi Pauli & Becky Burdine (Loeb 260)

6:30 91�������ד 7:30       Student/Faculty Challenge: Scavenger Hunt

7:30 91�������ד 10:00     Course BBQ (Loeb Quad)

10:00 91�������ד 11:30   Time-lapse microscopy: Andres Collazo & Jim Fadool (Group 4) (with Martin Distel91�������יs 36 hpf larvae, and any good ones from the morning91�������יs injected larvae)

10:00 91�������ד 11:30   Time-lapse microscopy: Jan Huisken and Dane Maxfield (Group 2) (with Martin Distel91�������יs 36 hpf larvae and any good ones from the morning91�������יs injected larvae)

Wed 30 Jul

8:30 91�������ד 12:00     Pit Talk & Lab: Adult dissections & tissue regeneration: Ken Poss (Loeb 260)

12:00 91�������ד 1:30     Faculty (Ken Poss and Holger Knaut) lunch with students (Swope)

1:30 91�������ד 5:00       Pit talk & Lab: Disease models: Martin Distel (Loeb 260)

6:00 91�������ד 7:00       Faculty Meeting (Memorial Circle)

7:00 91�������ד 10:00     Faculty & Staff Event; Student self-organized social time and/or assembly of images

Thurs 31 Jul

9:00 91�������ד 10:30      Workshop: Zebrafish in Education: Roshan Jain, Pierre Le Pabic, & Kara Cerveny (Loeb 263)

10:30 91�������ד 12:00    Pit Talk: Chimeric Analysis: Tom Schilling, Holger Knaut, & Jim Fadool (Loeb 260)

12:00 91�������ד 1:30      Faculty (Martin Distel) lunch with students (Swope)

1:30 91�������ד 6:00        Lab: Chimeric analysis 91�������ד Blastula transplants: Andreas Collazo, Jim Fadool, Holger Knaut, & Tom Schilling (Loeb 260)

7:30 91�������ד 9:00        Research Lectures & Discussion: The Ruth Sager Lectures in Genetics: Martin Distel & Kara Cerveny (Speck Auditorium)

9:00 91�������ד 11:00      91�������טDiSci91�������י (continued w speakers and all faculty present)

Fri 1 Aug

***Work on photo contest entries during 91�������טfree time91�������י today***

9:30 91�������ד 12:00     Lab: Imaging transplants: Andres Collazo, Jim Fadool, Holger Knaut, & Tom Schilling (Loeb 260)

9:30 91�������ד 10:30     Tour of the MBL Marine Resource Center (Groups 3 and 4)

12:00 91�������ד 1/1:30  Faculty (Kara Cerveny & Andres Collazo) lunch with students (Swope) (Groups 1 and 2: lunch till 1pm)

1/1:30 91�������ד 3:30    Lab: Disease modeling follow up: Martin Distel (Loeb 260) (Groups 1 and 2: start at 1; Groups 3 and 4: start at 1:30)

2:30 91�������ד 3:30       Tour of the MBL Marine Resource Center (Groups 1 and 2)

3:30 91�������ד 4:30       Review of time-lapses (Groups 1, 2, 3, 4): Jim Fadool, Andres Collazo, & Jan Huisken (Candle House 104)

4:45 91�������ד 5:30       Research Lecture & Discussion: Ken Poss (Speck Auditorium)

7:00 91�������ד 8:00       MBL Friday evening lecture (Clapp Auditorium) 91�������לNo Ordinary Vampire Fish: Insights from Sea Lampreys on Neuroscience and Human Health91�������ם (Jennifer Morgan, MBL Senior Scientist and Director of the Bell Center

9:00 91�������ד 11:00      91�������טDiSci91�������י (continued w speakers and all faculty present)

9:30 91�������ד 11:00      Additional imaging of transplants; assembly of images for transplant wrap-up

Sat 2 Aug

***Work on photo contest entries during 91�������טfree time91�������י today***

10:00-10:30      Wrap-up: Disease modeling (Martin Distel) (Clapp auditorium)

10:30 91�������ד 12:00   Workshop: Disease modeling: Martin Distel (Clapp auditorium)

12:00 91�������ד 1:30     Faculty (Kristen Kwan & Pierre Le Pabic) lunch with students (Swope)

1:30 91�������ד 6:00       Lab: Cardiac surgeries, Imaging of regenerating fin and heart: Ken Poss (Loeb 260)

7:30 91�������ד 9:00       Research Lectures & Discussion: Harry Burgess & Kristen Kwan (Speck Auditorium)

9:00 91�������ד 11:00     91�������טDiSci91�������י (continued w speakers and all faculty present)

9:30 91�������ד 11:00     Free Time prep for wrap up

Sun 3 Aug

***Work on photo contest entries during 91�������טfree time91�������י today***

8:30 91�������ד 10:00     Wrap-up: Chimeric analysis student presentations: Andres Collazo, Jim Fadool, Holger Knaut, & Tom Schilling (MRC 210)

10:00 91�������ד11:30    Pit Talk: Monitoring morphogenesis: Analysis of morphogenesis in 3D and 4D: Kristen Kwan (Loeb 260)

11:30 91�������ד 1:00     Faculty (Roshan Jain & Harry Burgess) lunch with students (Swope)

1:00 91�������ד 4:00       Lab: Monitoring morphogenesis: Kristen Kwan (Loeb 260)

4:00 91�������ד 5:00       Wrap-up of Monitoring morphogenesis: Kristen Kwan (Loeb 260)

5:00 91�������ד 6:30       Workshop: Responsible conduct of research: Tom Schilling & Andi Pauli (Candle House 104)

7:30 91�������ד 9:00       Chi-Bin Chien Memorial Research Lecture: Katie Drerup (Speck Auditorium), followed by reception (Tent in Loeb Quad)

10:00                Stony Beach evening quiet field trip to see bioluminescence (weather permitting)

Mon 4 Aug

***Work on five-minute student presentations of favorite data during 91�������טfree time91�������י today***

8:30 91�������ד 9:30       Pit Talk: Behavior: Harry Burgess & Jim Fadool (Loeb 260)

9:30 91�������ד 12:00     Lab: Behavior: Harry Burgess & Jim Fadool (Loeb 260)

12:00 91�������ד 1:30     Faculty (Katie Drerup) lunch with students (Swope)

1:30                  Photo contest images (individual & group entries) due by 1:30 pm

1:30 91�������ד 4:00       Lab, continued: Behavior: Harry Burgess & Jim Fadool (Loeb 260)

4:00 91�������ד 5:00       Wrap-up of Behavior: Harry Burgess & Jim Fadool (Loeb 260)

5:00 91�������ד 6:00       Workshop: Transgenesis: Sarah Kucenas (Candle House 104)

7:30 91�������ד 9:00       Research Lectures & Discussion: Sarah Kucenas & Jim Fadool (Speck Auditorium)

9:00 91�������ד 11:00     91�������טDiSci91�������י (continued w speakers and all faculty present)

10:00                Stony Beach evening quiet field trip to see bioluminescence (weather backup)

Tues 5 Aug

***Work on five-minute student presentations of favorite data during 91�������טfree time91�������י today***

8:30 91�������ד 9:30       Pit Talk: Optical Manipulation: Sarah Kucenas & Harry Burgess (Loeb 260)

9:30 91�������ד 12:30     Lab Rotations: Ablation/Axotomy & Optogenetics: Sarah Kucenas & Harry Burgess (Loeb 260)

12:30 91�������ד 1:30     Faculty (Sarah Kucenas & Jim Fadool) lunch with students (Swope)

1:30 91�������ד 3:30       Lab Rotations: Ablation/Axotomy & Optogenetics: Sarah Kucenas & Harry Burgess (Loeb 260) AND Fish facility tours with aquaculture vendors

3:30 91�������ד 4:30       Tea time with aquaculture vendors (Tent in Loeb Quad)

4:30 91�������ד 6:00       Work on 5-min presentations/free time

7:30 91�������ד 9:00       Research Lectures & Discussion: Andi Pauli & Tom Schilling (Speck Auditorium)

9:00 91�������ד 9:30       91�������טDiSci91�������י (continued w speakers and all faculty present)

9:30 91�������ד 10:30     Follow up on Ablation/Axotomy & Optogenetics: Sarah Kucenas & Harry Burgess (Candle House 104)

10:30 91�������ד 11        Finish work on five-minute student presentations of favorite data

11:00                Late-breaking photo contest images due by 11:00 pm

Wed 6 Aug

9:00 91�������ד 10:30     Lab: In vitro fertilization: April Freeman & Andi Pauli (Loeb 260)

10:30 91�������ד 12:00   Workshop: Zebrafish Husbandry, Breeding and Shipping: April Freeman (Loeb 260)

12:00 91�������ד 1:30     Lunch (Swope)

1:30 91�������ד 3:30       Five-minute student presentations of favorite data (Candle House 104)

3:30 91�������ד 5:00       Course Wrap-up Discussion: Tom Schilling & Andi Pauli (Tent in Loeb Quad)

5:00 91�������ד 5:15       Course Photo #1; wear course t-shirt (Loeb Courtyard)

6:00 91�������ד 10:00     Course Dinner and Student Awards with Course Photo #2 at 6:30 pm (Swope Lower Terrace)

Thurs 7 Aug

Breakfast, room checkout and departure